Studies on Single Cell Culture
نویسندگان
چکیده
منابع مشابه
Dynamic single cell culture array.
It is important to quantify the distribution of behavior amongst a population of individual cells to reach a more complete quantitative understanding of cellular processes. Improved high-throughput analysis of single cell behavior requires uniform conditions for individual cells with controllable cell-cell interactions, including diffusible and contact elements. Uniform cell arrays for static c...
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Rats were immunized with Salmonella adelaide flagella. By zone centrifugation of serum samples in sucrose gradients, it was shown that, as in many other systems of antibody formation, the first response was the formation of 19S, mercaptoethanol (ME)-sensitive antibody. This was quickly replaced by 7S, ME-insensitive antibody. Popliteal lymph node cell suspensions were prepared, and cells with a...
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Pneumonia virus of mice (PVM) has been serially propagated in a line of baby hamster kidney (BHK21) cells. A maximum titer of 6.3 x 10(6) TCID(50) per ml was obtained, and there was little variation in yield on serial passage. PVM grown in BHK21 cells was antigenically similar to virus obtained from the mouse lung, but was somewhat less virulent for the mouse after 10 serial passages in these c...
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A suspension culture technique for the study of peripheral blood cells from patients with leukemia is described. Cells from patients in relapse were preserved by freezing with dimethylsulfoxide at -70#{176}C to ensure the availability of the same population of cells for repeated experiments of varying designs. Cells from one AML patient proliferated only in the presence of phytohemagglutinin (P...
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The method for regeneration of plants from microspores was being developed for the last 50 years. Through the use of this method a tremendous progress in breeding of many important crop plants was achieved. This applies to cereals too. We have developed microspore culture techniques for many cereals and grasses, and here we recommend androgenesis as a tool to study morphological and biochemical...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1957
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.12.739